Vulgar Name: Human
|Age/Ethinicy:||78 years old|
|Derivation:||Established from undifferentiated thyroid carcinomas of a 78 year old female patient. Pathologically this primary carcinoma tissue contained residual well differentiated components suggesting well differentiated to undifferentiated carcinoma progression. Tumour suppresser genes p53, Rb, APC and MCC were analysed and sequence analysis confirmed a C:G to G:C transversion at the first base of p53 gene codon 248. Polymerase chain reaction-loss of heterozygosity assays confirmed allelic deletion of p53 gene. Loss of heterozygosity of tumour suppresser genes was not observed.|
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x 10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C. Saturation density at confluency is 1x100,000 cells/cm².
Doubling time is 36 hours.
Subcultivation ratio: 1:3 to 1:6 i.e. seeding at 2-4 x 10,000 cells/cm²
|Culture Conditions:||Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C|
|Cryopreservation:||95% FBS + 5% DMSO (Dimethyl sulfoxide)|
|Thawing Frozen Cells:||
|Depositors:||Etel Rodrigues Pereira Gimba - Instituto Nacional de Câncer|