Kyse-30

Code: 0404
Cell Line: Kyse-30
Species: Homo sapiens

Vulgar Name: Human
Tissue: Esophagus
Morphology: Epitheloid with long processes growing in monolayers
Disease: Squamous carcinoma
Growth Properties: Adherent
Sex: Male
Age/Ethinicy: 64-year-old; Asian
Derivation: KYSE-30 was established from the oesophageal cancer of an untreated 64 year old male. The tumour sample was taken from the mucosal surface of a well differentiated squamous cell carcinoma. The cell line KYSE-30 was established with the use of tumours initially transplanted to athymic mice. The cells are reported to have a doubling time of 20.8 hrs in the exponential growth phase. A p53 mutation at the splice acceptor site of intron 6 and a 12 fold amplification of c-erb B has been reported. KYSE-30 cells express a large number of epidermal growth factor receptors, 1.2x10,000,000 sites/cell.
Biosafey: 1
Additional info:
Culture Medium:
RPMI 1640 + Ham's F12 (1:1) + 2mM Glutamine + 2% Foetal Bovine Serum (FBS).

Subculturing:
Split sub-confluent cultures (70-80%) using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C



Medium Renewal: Every 2-6 days

Subcultivation ratio: 1:10 i.e. seeding at 1x10,000 cells/cm²
Culture Conditions: Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C
Cryopreservation: 95% FBS + 5% DMSO (Dimethyl sulfoxide)
Thawing Frozen Cells:
SAFETY PRECAUTION: Is highly recommend that protective gloves and clothing always be used and a full face mask always be worn when handling frozen vials. It is important to note that
some vials leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vessel exploding or blowing off its cap with dangerous force creating flying debris.
1. Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the Oring and cap out of the water. Thawing should be rapid (approximately 2 minutes).
2. Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.
3. For cells that are sensitive to DMSO is recommended that the cryoprotective agent be removed immediately. Transfer the vial contents to a centrifuge tube containing 9.0 mL complete culture medium and spin at approximately 125 x g for 5 to 7 minutes.
4.Discard the supernatant and Resuspend cell pellet with the recommended complete medium (see the specific batch information for the culture recommended dilution ratio).
5. Incubate the culture in a appropriate atmosphere and temperature (see "Culture Conditions" for this cell line).

NOTE: It is important to avoid excessive alkalinity of the medium during recovery of the cells. It is suggested that, prior to the addition of the vial contents, the culture vessel containing the growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6).

References:
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Characterization of 21 newly established esophageal cancer cell lines.
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PubMed=7913084; DOI=10.1002/ijc.2910580224
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Depositors: Olavo Bohrer Amaral-Universidade Federal do Rio de Janeiro