| BCRJ Code | 0042 |
| Cell Line | B 95-8 |
| Species | Saguinus oedipus |
| Vulgar Name | Monkey - Cotton-Top Tamarin |
| Tissue | Peripheral Blood |
| Cell Type | Lymphoblast |
| Morphology | Lymphoblast/Fibroblast |
| Growth Properties | Semiadherent |
| Derivation | Derived from a cotton-top tamarin (Saguinus oedipus). Releases high titres of transforming EBV. |
| Applications | Thus it provides a source of EBV to establish continuous lymphocytic lines from human donors. |
| Products | EBV |
| Biosafety | 2 |
| Addtional Info | The cells should be handled under laboratory containment level 2. In some instances the cell line B95-8 has been described as derived from ‘marmoset’, however, this is not correct. B95-8 was derived from a cotton-top tamarin (Saguinas oedipus); this has been confirmed by DNA profiling. |
| Culture Medium | RPMI-1640 medium modified to contain 2 mM L-glutamine, 1 mM sodium pyruvate, 4500 mg/L glucose and fetal bovine serum to a final concentration of 10%. |
| Subculturing | Subcultures are prepared by diluting the suspension. Cells on the floor of the flask may be dislodged by aspirating several times with culture medium or by rinsing with 0.25% trypsin - 0.53 mM EDTA solution. NOTE: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 12 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 6th edition, published by Alan R. Liss, N.Y., 2010. |
| Subculturing Medium Renewal | Every 2 to 3 days |
| Subculturing Subcultivation Ratio | 1:5 to 1:10 is recommended |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | Experientia 1996;52:818-826 - PMID: 8774755 Proc Natl Acad Sci USA 1972;69:383-387 - PMID: 4333982 Proc Natl Acad Sci USA 1973;70:190-194 - PMID: 4346033 |
| Depositors | Eliana Abdelay & Louise Calil Deterling, Universidade Federal do Rio de Janeiro. |
| Cellosaurus | CVCL_1953 |
