| BCRJ Code | 0360 |
| Cell Line | NCI-H1155 [H1155] |
| Species | Homo sapiens |
| Vulgar Name | Human |
| Tissue | Lung; Derived From Metastatic Site: Lymph Node |
| Morphology | Epithelial |
| Disease | Carcinoma; Non-Small Cell Lung Cancer |
| Growth Properties | Clusters in suspension |
| Sex | Male |
| Age/Ethinicity | 36 Year / Caucasian |
| Derivation | This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates from a lymph node metastasis obtained from a patient prior to therapy. |
| Products | Neuromedin B (NMB) |
| Biosafety | 1 |
| Addtional Info | The cells are able to synthesize the peptide NMB at 0.2 pmol/mg protein, but not the gastrin releasing peptide (GRP). |
| Culture Medium | DMEM: F-12 Medium contains 2.5 mM L-glutamine, 15 mM HEPES, 0.5 mM sodium pyruvate with: 0.02 mg/mL insulin 0.01 mg/mL transferrin 25 nM sodium selenite (final conc.) 50 nM Hydrocortisone (final conc.) 1 ng/mL Epidermal Growth Factor (do not filter) 0.01 mM ethanolamine (final conc.) 0.01 mM phosphorylethanolamine (final conc.) 100 pM triiodothyronine (final conc.) 0.5% (w/v) bovine serum albumin (final conc.) 0.5 mM sodium pyruvate (final conc.) extra 2mM L-glutamine (for final conc. of 4.5mM) |
| Subculturing | Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium. Add medium as the cell density increases. |
| Subculturing Medium Renewal | Every 2 to 3 days |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | Giaccone G, et al. Neuromedin B is present in lung cancer cell lines. Cancer Res. 52: 2732s-2736s, 1992. PubMed: 1563005 NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. |
| Depositors | Rui Manoel Reis - Hospital de Câncer de Barretos |
| Cellosaurus | CVCL_1456 |
