| BCRJ Code | 0380 |
| Cell Line | Y-79 |
| Species | Homo sapiens |
| Vulgar Name | Human |
| Tissue | Eye, retina |
| Cell Type | Retinoblastoma |
| Morphology | Multicellular clusters |
| Disease | Retinoblastoma |
| Growth Properties | Suspension |
| Sex | Female |
| Age/Ethinicity | 2 Year / Caucasian |
| Derivation | The Y79 line was isolated by T.W. Reid and associates in January 1971 by explant culture of a primary tumor from the right eye obtained immediately after enucleation. |
| Biosafety | 1 |
| Addtional Info | The donor had a strong maternal family history of retinoblastoma. Ultrastructural features including nuclear membrane infoldings, triple membrane structures, microtubules, large coated vesicles, centrioles, basal bodies and annulate lamellae were reportedly similar to those of the original tumor. |
| Culture Medium | RPMI-1640 medium modified to contain 2 mM L-glutamine, 4500 mg/L glucose with fetal bovine serum to a final concentration of 20%. |
| Subculturing | Allow aggregates to settle to the bottom of the flask. Remove supernatant and discard. Add fresh medium, aspirate and dispense into new flasks. |
| Subculturing Medium Renewal | Twice per week |
| Subculturing Subcultivation Ratio | 1:2 to 1:4 is recommended |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | Reid TW, et al. Characteristics of an established cell line of retinoblastoma. J. Natl. Cancer Inst. 53: 347-360, 1974. PubMed: 4135597 Rostomily RC, et al. Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res. 57: 3526-3531, 1997. PubMed: 9270024 Wong HK, Ziff EB. The human papillomavirus type 16 E7 protein complements adenovirus type 5 E1A amino-terminus-dependent trasactivation of adenovirus type 5 early genes and increases ATF and Oct-1 DNA binding activity. J. Virol. 70: 332-340, 1996. PubMed: 8523545 |
| Depositors | Silvia de Toledo - Grupo de Apoio ao Adolescente e a Criança com Câncer |
| Cellosaurus | CVCL_1893 |
