| BCRJ Code | 0381 |
| Cell Line | SK-N-BE(2) |
| Species | Homo sapiens |
| Vulgar Name | Human |
| Tissue | Brain |
| Cell Type | Neuroblast |
| Morphology | neuroblast |
| Disease | Neuroblastoma |
| Growth Properties | Mixed, Adherent And Suspension |
| Sex | Male |
| Age/Ethinicity | 2 Year / |
| Derivation | derived from metastatic site: bone marrow |
| DNA Profile | Amelogenin: X,Y CSF1PO: 10 D13S317: 11 D16S539: 9,11 D5S818: 12 D7S820: 9,10 THO1: 6,7 TPOX: 8,11 vWA: 18 |
| Biosafety | 1 |
| Addtional Info | The SK-N-BE(2) neuroblastoma cell line was established in November of 1972 from a bone marrow biopsy taken from child with disseminated neuroblastoma after repeated courses of chemotherapy and radiotherapy. Population dubling time: 30h The cells exhibit moderate levels of dopamine beta hydroxylase activity. SK-N-BE(2) cells have a reported saturation density greater than 1 X 106 cells/cm2. The morphology of the cells varies with some cells having long processes and others that are epithelioid like. The cells will aggregate, form clumps and float. |
| Culture Medium | 1:1 mixture of Eagle's Minimum Essential Medium and F12 Medium fetal bovine serum to a final concentration of 10%. |
| Subculturing | These cells grow as a mixture of floating and adherent cells. Remove the medium with the floating cells, and recover the cells by centrifugation. Rinse the adherent cells with a fresh 0.25% trypsin, 0.53 mM EDTA solution, add an additional 1 to 2 mL of trypsin solution, and let the culture sit at room temperature (or at 37°C) until the cells detach. Add fresh medium, aspirate, combine with the floating cells recovered above and dispense into new flasks. |
| Subculturing Medium Renewal | 1:12 to 1:20 is recommended. |
| Subculturing Subcultivation Ratio | Every 4 to 7 days. |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | Biedler JL, Spengler BA. A novel chromosome abnormality in human neuroblastoma and antifolate-resistant Chinese hamster cell lives in culture. J. Natl. Cancer Inst. 57: 683-695, 1976. PubMed: 62055 Barnes EN, et al. The fine structure of continuous human neuroblastoma lines SK-N-SH, SK- N-BE(2), and SK-N-MC. In Vitro 17: 619-131, 1981. PubMed: 7327593 Biedler JL, Spengler BA. Metaphase chromosome anomaly: association with drug resistance and cell-specific products. Science 191: 185-187, 1976. PubMed: 942798 Biedler JL, et al. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 38: 3751-3757, 1978. PubMed: 29704 |
| Depositors | Silvia de Toledo - Grupo de Apoio ao Adolescente e a Criança com Câncer |
| ATCC | CRL-2271 |
| Cellosaurus | CVCL_0528 |
