| BCRJ Code | 0001 |
| Cell Line | 11B11 [BSF-1] |
| Species | Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Vulgar Name | Mouse / Rat |
| Cell Type | Hybridoma: B Lymphocyte |
| Morphology | Lymphoblast |
| Growth Properties | Suspension |
| Derivation | Animals were immunized with purified BSF-1 emulsified in complete Freund's adjuvant. Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells. |
| Applications | The antibody does not cross-react with other lymphokines, and can be used to purify BSF-1 from complex mixtures. |
| Products | immunoglobulin; monoclonal antibody; against murine B cell stimulatory factor 1 (BSF-1, interleukin-4, interleukin 4, IL-4, B cell growth factor 1, BCGF-1) |
| Biosafety | 1 |
| Culture Medium | RPMI 1640 medium with L-glutamine and 10% of heat-inactivated fetal bovine serum. |
| Subculturing | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 10e5 viable cells/mL. Maintain cultures at a cell concentration between 1 x 10e5 and 1 x 10e6 cells/mL. NOTE: Do not allow the cell concentration to exceed 1 x 10e6 cells/mL. |
| Subculturing Medium Renewal | Every 2 to 3 days |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | Ohara J, Paul WE. Production of a monoclonal antibody to and molecular characterization of B-cell stimulating factor-1. Nature 315: 333-336, 1985. PubMed: 2582266 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |
| Depositors | Ises A. Abrahamsohn, Universidade de São Paulo |
| Cellosaurus | CVCL_9139 |
