Catálogo de Células

0004 - 1E8

.
BCRJ Code 0004
Cell Line 1E8
Species Mus musculus
Vulgar Name Mouse; Balb/C
Tissue Dorsal Root Ganglia
Cell Type Hybridom: B Lymphocyte
Morphology Lymphoblast
Disease Myeloma
Growth Properties Suspension
Sex Female
Biosafety 1
Culture Medium Iscove's Modified Dulbecco's Medium (IMDM) contains 4 mM L-glutamine, 4500 mg/L glucose and 20% of fetal bovine serum.
Subculturing Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 10e5 viable cells/mL. Maintain cultures at a cell concentration between 1 x 10e5 and 1 x 10e6 cells/mL. NOTE: Do not allow the cell concentration to exceed 1 x 10e6 cells/mL.
Subculturing Medium Renewal Every 2 to 3 days
Culture Conditions Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C
Cryopreservation 95% FBS + 5% DMSO (Dimethyl sulfoxide)
Thawing Frozen Cells SAFETY PRECAUTION: It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
  1. Thaw the vial by gently agitating it in a 37°C water bath. To minimize contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 2 minutes).
  2. Remove the vial from the water bath as soon as its contents are thawed and decontaminate it by dipping in or spraying with 70% ethanol. From this point, all operations must be performed under strict aseptic conditions.
  3. For cells sensitive to DMSO, it is recommended to remove the cryoprotective agent immediately. Transfer the vial contents to a centrifuge tube containing 9.0 mL of complete culture medium and centrifuge at approximately 125 × g for 5 to 7 minutes.
  4. Discard the supernatant and resuspend the cell pellet in the recommended complete medium (see specific batch information for the appropriate dilution ratio).
  5. Incubate the culture under appropriate atmospheric and temperature conditions (see "Culture Conditions" for this cell line).

NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6).
References Bhattacharya, A., Frank, E., Ratner, N., and Brackenbury, R.(1991) is an early marker of the Schwann cell lineage in chickens. Neuron 7, 831-844. Robb, S.M.C., and Sanchez Alvarado, A. (2002). Identification of immunological reagents for use in the study of freshwater planarians by means of whole-mount immunofluorescence and confocal microscopy. Genesis 32, 293-298.
Depositors Andréa Gonçalves Trentini – Universidade Federal de Santa Catarina
Cellosaurus CVCL_D141
Contatos

Fale com o BCRJ

Outras tecnologias foram desenvolvidas dentro do BCRJ repassadas para o mercado produtivo e hoje estão em pleno funcionamento.

O BCRJ

Contatos

Redes Sociais

Instagram Linkedin Facebook Youtube

RioMarca Agência Web