| BCRJ Code | 0333 |
| Cell Line | 32D clone 3 |
| Species | Mus muscullus |
| Vulgar Name | Mouse |
| Tissue | Bone Marrow |
| Morphology | Lymphoblast |
| Growth Properties | Mixed, Adherent And Suspension |
| Applications | This cell line is a suitable transfection host. |
| Biosafety | 1 |
| Addtional Info | This line is IL-3 dependent. |
| Culture Medium | RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 4.5 g/L glucose, 10% of heat-inactivated fetal bovine serum and 10% mouse Interleukin-3 culture supplement. |
| Subculturing | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 105 viable cells/mL. Maintain cell density between 2 X 105 and 1 X 106 viable cells/mL. Scrape off the attached cells and transfer along with the floating cells into new flasks. NOTE: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 12 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 6th edition, published by Alan R. Liss, N.Y., 2010. |
| Subculturing Medium Renewal | Every 2 to 3 days |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | Ahmed N, Berridge MV. Regulation of glucose transport by interleukin-3 in growth factor-dependent and oncogene-transformed bone marrow-derived cell lines. Leuk. Res. 21: 609-618, 1997. PubMed: 9301681 Ahmed N, Berridge MV. Distinct regulation of glucose transport by interleukin-3 and oncogenes in a murine bone marrow-derived cell line. Biochem. Pharmacol. 57: 387-396, 1999. PubMed: 9933027 |
| Depositors | Fábio Santos - Hospital Israelita Albert Einstein |
| Cellosaurus | CVCL_0119 |
