| BCRJ Code | 0398 |
| Cell Line | 8505c |
| Species | Homo sapiens |
| Vulgar Name | Human |
| Tissue | Thyroid |
| Cell Type | Epithelial |
| Morphology | Epithelial |
| Disease | Carcinoma, undifferentiated |
| Growth Properties | Adherent |
| Sex | Female |
| Age/Ethinicity | 78 Year / |
| Derivation | Established from undifferentiated thyroid carcinomas of a 78 year old female patient. Pathologically this primary carcinoma tissue contained residual well differentiated components suggesting well differentiated to undifferentiated carcinoma progression. Tumour suppresser genes p53, Rb, APC and MCC were analysed and sequence analysis confirmed a C:G to G:C transversion at the first base of p53 gene codon 248. Polymerase chain reaction-loss of heterozygosity assays confirmed allelic deletion of p53 gene. Loss of heterozygosity of tumour suppresser genes was not observed |
| Biosafety | 1 |
| Culture Medium | Dulbecco's Modified Eagle's Medium (DMEM) with + 2 mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% of Fetal Bovine Serum (FBS). |
| Subculturing | Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x 10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C. Saturation density at confluency is 1x100,000 cells/cm². Doubling time is 36 hours. |
| Subculturing Subcultivation Ratio | 1:3 to 1:6 i.e. seeding at 2-4 x 10,000 cells/cm² |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | Cancer Res 1992;52:1369; Int J Oncology 1994;4:583 |
| Depositors | Etel Rodrigues Pereira Gimba - Instituto Nacional de Câncer |
| Cellosaurus | CVCL_1054 |
