| BCRJ Code | 0311 |
| Cell Line | AGS |
| Species | Homo sapiens |
| Vulgar Name | Human |
| Tissue | Stomach |
| Cell Type | Epithelial |
| Morphology | Epithelial |
| Disease | Gastric Adenocarcinoma |
| Growth Properties | Adherent |
| Sex | Female |
| Age/Ethinicity | 54 Year / Caucasian |
| Derivation | THE AGS CELL LINE WAS DERIVED FROM FRAGMENTS OF A BIOPSY SPECIMEN OF NA UNTREATED HUMAN ADENOCARCINOMA OF STOMACH. |
| Applications | This cell line is a suitable transfection host. |
| Tumor Formation: | YES, IN ATHYMIC BALB/C MICE |
| Biosafety | 2 |
| Addtional Info | THIS CELL LINE MAY RELEASE PARAINFLUENZAVIRUS TYPE 5 (FORMELY KNOWN AS SIMIAN VIRUS 5). THE VIRUS INTERFERES WITH INTERFERON-SIGNALLING WITHIN THE CELL LINE BY DEGRADATION OF STAT1. |
| Culture Medium | Dulbecco's Modified Eagle's Medium (DMEM) modified to contain 2mM L-glutamine, 4500 mg/L glucose and fetal bovine serum to a final concentration of 10%. |
| Subculturing | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks) to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Population Doubling Time: 20 hrs NOTE: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 12 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 6th edition, published by Alan R. Liss, N.Y., 2010. |
| Subculturing Medium Renewal | Every 2 to 3 days |
| Subculturing Subcultivation Ratio | A ratio of 1:2 to 1:6 is recommended |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | BARRANCO SC ET AL. ESTABLISHMENT AND CHARACTERIZATION OF NA IN VIVO MODEL SYSTEM FOR HUMAM ADENOCARCINOMA OF THE STOMACH. CANCER RES 43:1703-9, 1982 |
| Depositors | MARCELO LIMA RIBEIRO - UNIFAG |
| Cellosaurus | CVCL_0139 |
