| BCRJ Code | 0059 |
| Cell Line | Caco-2 |
| Species | Homo sapiens |
| Vulgar Name | Human |
| Tissue | Colon |
| Cell Type | Epithelial |
| Morphology | Epithelial |
| Disease | Coloretal Adenocarcinoma |
| Growth Properties | Adherent |
| Sex | Male |
| Age/Ethinicity | 72 Year / Caucasian |
| Applications | This cell line is a suitable transfection host. |
| DNA Profile | Amelogenin: X CSF1PO: 11 D13S317: 11,13,14 D16S539: 12,13 D5S818: 12,13 D7S820: 11,12 THO1: 6 TPOX: 9,11 vWA: 16,18 |
| Virus Resistance: | Human immunodeficiency virus 1 , Human immunodeficiency virus 1 |
| Tumor Formation: | Yes, in nude mice; forms moderately well differentiated adenocarcinoma consistent with colonic primary (grade II) |
| Products | keratin retinoic acid binding protein 1 retinol binding protein 2 |
| Biosafety | 1 |
| Addtional Info | Upon reaching confluence, the cells express characteristics of enterocytic differentiation [PubMed: 1939345]. Caco-2 cells express retinoic acid binding protein I and retinol binding protein II [PubMed: 9040537] |
| Culture Medium | Dulbecco's Modified Eagle's Medium (DMEM) with 1% non-essential amino acids, 2 mM L-glutamine, 1 mM sodium pyruvate, 1.0 g/L glucose and 20% of fetal bovine serum. |
| Subculturing | NOTE: Subculture cells when they are about 80%, confluent at a cell concentration between 8 x 10e4 and 1 x 10e5 cell/cm2. Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. T-75 flasks are recommended for subculturing this product. Remove and discard culture medium. Briefly rinse the cell layer with PBS without calcium and magnesium to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.The recommended inoculum is 1 X 10Ee4 viable cells/cm2. Incubate cultures at 37°C. Population Doubling Time about: 62 hours NOTE: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 12 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 6th edition, published by Alan R. Liss, N.Y., 2010. |
| Subculturing Medium Renewal | 1 to 2 times per week |
| Subculturing Subcultivation Ratio | 1:4 to 1:6 |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | J. Natl. Cancer Inst. 58: 209-214, 1977; J. Natl. Cancer Inst. 59: 221-226, 1977. |
| Depositors | Dr. Joao R. C. Andrade, Universidade do Estado do Rio de janeiro. Cláudia Maria Oliveira Simões, Universidade Federal de Santa Catarina. CRISTINA MASSOCO- CIALLYX LÚCIO MENDES CABRAL - Universidade Federal do Rio de Janeiro Aldo Angelo Moreira |
| ATCC | HTB-37 |
| Cellosaurus | CVCL_0025 |
