| BCRJ Code | 0408 |
| Cell Line | CAKI 2 |
| Species | Homo sapiens |
| Vulgar Name | Human |
| Tissue | Kidney |
| Cell Type | Epithelial |
| Morphology | Epithelial |
| Disease | Clear cell Carcionoma |
| Growth Properties | Adherent |
| Sex | Male |
| Age/Ethinicity | 69 Year / White |
| Applications | 3D cell culture; Cancer research |
| Tumor Formation: | Yes, in nude mice; forms clear cell carcinoma |
| Products | Antigen expression: Blood Type A; Rh- Isoenzymes: AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1-2 Me-2, 1 PGM1, 1 PGM3, 1 |
| Biosafety | 1 |
| Culture Medium | McCoy's 5a + 2mM Glutamine + 10% of Fetal Bovine Serum (FBS). |
| Subculturing | Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow he flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. |
| Subculturing Medium Renewal | 2 to 3 times per week |
| Subculturing Subcultivation Ratio | 1:3 to 1:6 |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | Fogh J. Human tumor cells in vitro. New York: Plenum Press; 1975. Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034 Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047 Kovacs G, et al. The Heidelberg classification of renal cell tumors. J. Pathol. 183: 131-133, 1997. PubMed: 9390023 |
| Depositors | Banco de Células do Rio de Janeiro |
| Cellosaurus | CVCL_0235 |
