| BCRJ Code | 0264 |
| Cell Line | Calu-3 |
| Species | Homo sapiens |
| Vulgar Name | Human |
| Tissue | Lung Adenocarcinoma; Derived From Metastatic Site: Pleural Effusion |
| Cell Type | Epithelial |
| Morphology | Epithelial |
| Disease | Adenocarcinoma |
| Growth Properties | Adherent |
| Sex | Male |
| Age/Ethinicity | 25 Year / Caucasian |
| Applications | This cell line is a suitable transfection host. |
| DNA Profile | Amelogenin: X CSF1PO: 11,12 D13S317: 12 D16S539: 12,14 D5S818: 11 D7S820: 10,11 THO1: 6,9.3 TPOX: 8 vWA: 16,17 |
| Tumor Formation: | Yes, forms well differentiated grade I adenocarcinoma in nude mice |
| Products | Antigen expression: Blood Type A; Rh+ |
| Biosafety | 1 |
| Addtional Info | The patient had received prior therapy with cytoxan, bleomycin and adriamycin. |
| Culture Medium | Dulbecco's Modified Eagle's Medium (DMEM) with 1% non-essential amino acids, 2 mM L-glutamine, 1 mM sodium pyruvate, 1.0 g/L glucose and 20% of fetal bovine serum. |
| Subculturing | Remove and discard culture medium. Briefly rinse the cell layer with PBS without calcium and magnesium to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. T-75 flasks are recommended for subculturing this product. NOTE: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 12 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 6th edition, published by Alan R. Liss, N.Y., 2010. |
| Subculturing Medium Renewal | 2 to 3 times per week |
| Subculturing Subcultivation Ratio | 1:3 to 1:6 |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | 21869: . Human tumor cells in vitro. New York: Plenum Press; 1975. 22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080 24381: Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047 |
| Depositors | Eliana Martins Lima, Universidade Federal de Goiás |
| ATCC | HTB-55 |
| Cellosaurus | CVCL_0609 |
