BCRJ Code | 0400 |
Cell Line | CCD-18Co |
Species | Homo sapiens |
Vulgar Name | Human |
Tissue | Colon |
Morphology | Fibroblast |
Disease | Normal |
Growth Properties | Adherent |
Sex | Female |
Age/Ethinicity | 2 Month / Black |
Biosafety | 1 |
Addtional Info | The line begins to senesce at about PDL = 42. |
Culture Medium | DMEM Low glucose + fetal bovine serum to a final concentration of 10%. |
Subculturing | Remove medium, and rinse with 0.25% trypsin, 0.053 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Note: Growth of the cells is enhanced by addition of tumor necrosis factor alpha (TNF alpha) to the medium. |
Subculturing Medium Renewal | Every 2 to 3 days |
Subculturing Subcultivation Ratio | 1:2 to 1:3 |
Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
References | Sugarman BJ, et al. Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro. Science 230: 943-945, 1985. PubMed: 3933111 Hinterleitner TA, et al. Il-1 stimulates intestinal myofibroblast COX gene expression and augments activation of CI- secretion in T84 cells. Am. J. Physiol. 271: C1262-C1268, 1996. PubMed: 8897833 |
Depositors | Heitor Souza - UFRJ |
Cellosaurus | CVCL_2379 |
Fale conosco: