| BCRJ Code | 0064 |
| Cell Line | CEC-32 |
| Species | Coturnix japonica |
| Vulgar Name | Quail |
| Tissue | Embryo |
| Cell Type | Fibroblast |
| Morphology | Fibroblast |
| Growth Properties | Adherent |
| Derivation | Problematic cell line: Misidentified. Originally thought to be of chicken origin but found to be from quail (PubMed=10954914). |
| Products | Interfereon regulatory factor 1 after stimulation with interferon (Jungwirth et al., 1995). |
| Biosafety | 1 |
| Culture Medium | Dulbecco's Modified Eagle's Medium (DMEM) with 1.0 g/L glucose and 10% of fetal bovine serum. |
| Subculturing | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. T-75 flasks are recommended for subculturing this product. Remove and discard culture medium. Briefly rinse the cell layer with PBS without calcium and magnesium to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. NOTE: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 12 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 6th edition, published by Alan R. Liss, N.Y., 2010. |
| Subculturing Medium Renewal | Every 2 to 3 days |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | PubMed=6293961; DOI=10.1007/BF02796323. Kaaden, O. R., Lange, S., Stiburek, B., 1982. Establishment and characterization of chicken embryo fibroblast clone LSCC-H32. In vitro 18:827-834(1982) PubMed=18766641; DOI=10.1080/03079458708436402 - Nazerian K. An updated list of avian cell lines and transplantable tumours. Avian Pathol. 16:527-544(1987) PubMed=10954914; DOI=10.1089/10799900050116417 Zoller B., Redman-Muller I., Nanda I., Guttenbach M., Dosch E., Schmid M., Zoorob R., Jungwirth C. Sequence comparison of avian interferon regulatory factors and identification of the avian CEC-32 cell as a quail cell line. J. Interferon Cytokine Res. 20:711-717(2000) Zoller, B., Redman-Muller, I., Nanda, I., Guttenbach, M., Dosch, E., Schmid, M., Zoorob, R., Jungwirth, C., 2000. Sequence comparison of avian interferon regulatory factors and identification of the avian CEC-32 cell as quail cell line. J. Interf. Cytok. Res. 20, 711-717. Jungwirth, C., M. Rebbert, K. Ozato, H. J. Degen, U. Schultz, I. B. Dawid, 1995. Chicken interferon consensus sequence-binding protein (ICSBP) and interferon regulatory factor (IRF) 1 genes reveal evolutionary conservation in the IRF gene family Proc. Natl. Acad. Sci. USA 92, 3105-3109. |
| Depositors | Fabiana Horn, Universidade Federal do Rio Grande do Sul. |
| Cellosaurus | CVCL_D160 |
