| BCRJ Code | 0268 |
| Cell Line | D1-4G2-4-15 |
| Species | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Vulgar Name | Mouse |
| Tissue | Spleen |
| Cell Type | B Lymphocyte; Hybridoma |
| Morphology | Lymphoblast-Like |
| Growth Properties | Suspension |
| Derivation | Animals were immunized with dengue virus type 2 antigens. Spleen cells were fused with P3X63Ag8 myeloma cells. |
| Products | immunoglobulin; antibody against flavivirus group antigen |
| Biosafety | 1 |
| Addtional Info | The antibody reacts with flavivirus group specific antigens. |
| Culture Medium | Hybri-Care Medium from ATCC (Catalog No. 46-X) and fetal bovine serum to a final concentration of 10%. |
| Subculturing | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 10e5 viable cells/mL. Maintain cultures at a cell concentration between 2 x 10e5 and 1 x 10e6cells/mL. |
| Subculturing Medium Renewal | 2 to 3 times a week |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | 922: Henchal EA, et al. Dengue virus-specific and Flavivirus group determinants identified with monoclonal antibodies by indirect immunofluorescence. Am. J. Trop. Med. Hyg. 31: 830-836, 1982. PubMed: 6285749 |
| Depositors | Danielle da Glória D Souza, Universidade Federal de Minas Gerais |
| ATCC | HB-112 |
| Cellosaurus | CVCL_J890 |
