| BCRJ Code | 0084 |
| Cell Line | F23.2 |
| Species | Rattus norvegicus |
| Vulgar Name | Rat |
| Tissue | Blood |
| Cell Type | Hybridoma: B Lymphocyte |
| Morphology | Lymphoblast |
| Growth Properties | Suspension |
| Products | Immunoglobulin; monoclonal antibody; TcR murine: ab against; V-beta 8.2: ab against |
| Biosafety | 1 |
| Addtional Info | This hybridoma secretes monoclonal antibody agaisnt TcR murine (V-beta 8.1, 8.2, 8.3 molecules ). The origin of this cell line should be acknowledged in all relevant publication |
| Culture Medium | RPMI-1640 medium modified to contain 2 mM L-glutamine, 1 mM sodium pyruvate, 4500 mg/L glucose and fetal bovine serum to a final concentration of 10%. |
| Subculturing | Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml. Maintain cultures from 1e10E5 to 1x10E06 cells/mL. |
| Subculturing Medium Renewal | 2 to 3 times per week |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| Depositors | Alberto Nobrega, Universidade Federal do Rio de Janeiro |
| Cellosaurus | CVCL_D666 |
