| BCRJ Code | 0086 |
| Cell Line | FDC-P1 |
| Species | Mus musculus |
| Vulgar Name | Mouse; Dba/2 |
| Tissue | Bone Marrow |
| Morphology | Lymphoblast |
| Disease | Normal |
| Growth Properties | Suspension |
| Derivation | The FDC-P1 cell line was established from long term culture of normal DBA/2 bone marrow cells in medium conditioned by WEHI-3 cells. |
| Applications | This cell line is a suitable transfection host. |
| Biosafety | 1 |
| Addtional Info | The FDCP-1 cell line is dependent upon IL-3 or GM-CSF or WEHI-3 conditioned medium (WEHI-3CM) for continued growth. It can be used to quantify the presence of these growth factors in biological fluids. |
| Culture Medium | Dulbecco's modified Eagle's medium with 4 mM L-glutamine with 4.5 g/L glucose, 10% of fetal bovine serum and 25% mouse Interleukin-3 culture supplement. (Supplement purchased from BD Biosciences, Catalog No. 354040). |
| Subculturing | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 10e5 viable cells/mL. Maintain cultures at a cell concentration between 1 x 10e5 and 1 x 10e6 cells/mL. NOTE: Do not allow the cell concentration to exceed 1 x 10e6 cells/mL. Population Doubling Time about: 24-30 hours |
| Subculturing Medium Renewal | Every 2 to 3 days |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | Dexter TM, et al. Growth of factor-dependent hemopoietic precursor cell lines. J. Exp. Med. 152: 1036-1047, 1980. PubMed: 6968334 |
| Depositors | Evan secor, USA. |
| Cellosaurus | CVCL_2039 |
