| BCRJ Code | 0099 |
| Cell Line | HD-11 |
| Species | Gallus gallus |
| Vulgar Name | Chicken |
| Tissue | Bone Marrow |
| Cell Type | Macrophage |
| Morphology | Macrophage |
| Disease | Leukemia |
| Growth Properties | Adherent |
| Sex | Female |
| Derivation | Bone marrow cells Chicken transformed by avian leukemia virus MC29 (Beug et al., 1979). |
| Products | This cells are recognized by monoclonal antibody anti-macrophage of chicken KUL01 (Mast et al, 1998). |
| Biosafety | 1 |
| Addtional Info | Bone marrow cells of galine transformed by avian leukemia virus. |
| Culture Medium | RPMI-1640 medium modified with 4500 mg/L glucose and 10% of fetal bovine serum. |
| Subculturing | Remove and discard culture medium. Briefly rinse the cell layer with PBS without calcium and magnesium to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. NOTE: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 12 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 6th edition, published by Alan R. Liss, N.Y., 2010. |
| Subculturing Medium Renewal | Every 2 to 3 days |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | Beug, H., A. von Kirchbach, G. Döderlein, J. -F. Conscience, T. Graf. (1979). Chiken hematopoietic cells transformed by seven strains of defective avian leukemia viruses display three distinct phenotypes of differentiation. Cell 18: 375-90. Mast, J., B.M. Goddeeris, K. Peeters, F. Vandesandle, L.R. Berghman (1998). Characterisation of chicken monocytes, macrophages and interdigitating cells by the monoclonal antibody KUL01. Vet. Imunol. Immunopathol. 61: 343-357. |
| Depositors | Fabiana Horn, Universidade Federal do Rio Grande do Sul. |
| Cellosaurus |
