| BCRJ Code | 0308 |
| Cell Line | HEL92.1.7 |
| Species | Homo sapiens |
| Vulgar Name | Human |
| Tissue | Bone Marrow |
| Cell Type | Erythroblast |
| Morphology | Lymphoblast |
| Disease | Erythroleukemia |
| Growth Properties | Suspension |
| Sex | Male |
| Age/Ethinicity | 30 Year / Caucasian |
| DNA Profile | Amelogenin: X,Y CSF1PO: 10 D13S317: 9,11 D16S539: 11 D5S818: 11 D7S820: 7 THO1: 7 TPOX: 11 vWA: 14,17 |
| Products | Genes Expressed: hemoglobin; globin (G gamma, A gamma, epsilon, eta and alpha chains); beta-2-microglobulin; glycophorin |
| Biosafety | 1 |
| Addtional Info | These cells differentiate spontaneously into erythroblast-like cells. Macrophage-like differentiation can be induced with phorbol esters such as TPA (12-O-tetradecanoyl-phorbol-13-acetate) and PMA (phorbol myristic acid). Antigen expression: HLA A3, Aw32, Bw35; Ia+ |
| Culture Medium | RPMI-1640 medium modified to contain 2 mM L-glutamine, 4500 mg/L glucose and 10% of fetal bovine serum. |
| Subculturing | Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10e5 cells/mL and maintain between 1 x 10e5 and 1 x 10e6 cells/mL. |
| Subculturing Medium Renewal | Every 2 to 3 days |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | Papayannopoulou T, et al. Human erythroleukemia cell line (HEL) undergoes a drastic macrophage- like shift with TPA. Blood 62: 832-845, 1983 |
| Depositors | RENATO JOSÉ S. OLIVEIRA - HOSPITAL DE CÂNCER DE BARRETOS |
| Cellosaurus | CVCL_2481 |
