| BCRJ Code | 0310 |
| Cell Line | HGC-27 |
| Species | Homo sapiens |
| Vulgar Name | Human |
| Tissue | Stomach |
| Cell Type | Epithelial; Polygonal Or Short Spindle-Shaped |
| Morphology | Epithelial |
| Disease | Gastric Carcinoma |
| Growth Properties | Adherent |
| Derivation | This cell line was established by culture of the metastatic lymph node from a gastric cancer patient diagnosed histological as undifferentiated carcinoma. |
| Tumor Formation: | YES |
| Biosafety | 1 |
| Culture Medium | Dulbecco's Modified Eagle's Medium (DMEM) with 2 mM L-glutamine, 1.0 g/L glucose and 10% of fetal bovine serum. |
| Subculturing | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Trypsin-EDTA (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. Doubling Time: 17 hrs NOTE: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 12 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 6th edition, published by Alan R. Liss, N.Y., 2010. |
| Subculturing Medium Renewal | Every 2 to 3 days |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | AKAGI, T, KIMOTO T. HUMAN CELL LINE (HGC-27) DERIVED FROM METASTATIC LYMPH NODE OF GASTRIC CANCER. ACTA MED OKAYAMA 30(3): 215-219, 1974 |
| Depositors | MARCELO LIMA RIBEIRO - UNIFAG |
| Cellosaurus | CVCL_1279 |
