| BCRJ Code | 0377 |
| Cell Line | IMR-32 |
| Species | Homo sapiens |
| Vulgar Name | Human |
| Tissue | Brain; derived from metastatic site: abdominal mass |
| Cell Type | Neuroblast |
| Morphology | Fibroblast; neuroblast |
| Disease | Neuroblastoma |
| Growth Properties | Adherent |
| Sex | Male |
| Age/Ethinicity | 13 Month / |
| Derivation | The IMR-32 cell line was established by W.W. Nichols, J. Lee and S. Dwight in April, 1967 from an abdominal mass occurring in a 13-month-old Caucasian male. The tumor was diagnosed as a neuroblastoma with rare areas of organoid differentiation. |
| Applications | This cell line is a suitable transfection host. |
| Virus Succeptility: | Vesicular stomatitis, Orsay (Indiana) Vesicular stomatitis, Glasgow (Indiana) Herpes simplex virus Vaccinia virus Human Coxsackievirus B3 Human poliovirus 3 |
| Biosafety | 1 |
| Addtional Info | Two cell types are present. Predominant is a small neuroblast-like cell.The other is a large hyaline fibroblast. IMR-32 cells may pile up and grow in patches. IMR-32 cells may not become 100% confluent. |
| Culture Medium | Dulbecco's Modified Eagle's Medium (DMEM) with 2 mM L-glutamine and fetal bovine serum to a final concentration of 10%. |
| Subculturing | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with PBS without calcium and magnesium to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until the cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Transfer cell suspension to centrifuge tube and spin at approximately 125 x g for 5 to 10 minutes. Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels. Maintain cultures at a cell concentration between 4x104 and 4 x 105 cells/cm2. Place culture vessels in incubators at 37°C. NOTE: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 12 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 6th edition, published by Alan R. Liss, N.Y., 2010. Population Doubling Time: approximately 20 hrs. |
| Subculturing Medium Renewal | Every 2 to 3 days |
| Subculturing Subcultivation Ratio | 1:3 to 1:6 is recommended |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | Tumilowicz JJ, et al. Definition of a continuous human cell line derived from neuroblastoma. Cancer Res. 30: 2110-2118, 1970. PubMed: 5459762 Rostomily RC, et al. Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res. 57: 3526-3531, 1997. PubMed: 9270024 Maestrini E, et al. A family of transmembrane proteins with homology to the MET-hepatocyte growth factor receptor. Proc. Natl. Acad. Sci. USA 93: 674-678, 1996. PubMed: 8570614 |
| Depositors | Silvia de Toledo - Grupo de Apoio ao Adolescente e a Criança com Câncer |
| Cellosaurus | CVCL_0346 |
