| BCRJ Code | 0118 |
| Cell Line | IMR-90 |
| Species | Homo sapiens |
| Vulgar Name | Human |
| Tissue | Lung |
| Cell Type | Fibroblast |
| Morphology | Fibroblast |
| Disease | Normal |
| Growth Properties | Adherent |
| Sex | Female |
| Age/Ethinicity | 16 (gestation) Week / Caucasian |
| Applications | This cell line is a suitable transfection host. |
| DNA Profile | Amelogenin: X CSF1PO: 11,13 D13S317: 11,13 D16S539: 10,13 D5S818: 12,13 D7S820: 9,12 THO1: 8,9.3 TPOX: 8,9 vWA: 16,19 |
| Virus Succeptility: | Human poliovirus 1 Human poliovirus 2 Varicella-Zoster Herpes simplex virus 1 Herpes simplex virus 2 Human poliovirus 3 Vaccinia virus Human herpesvirus 5 , Human cytomegalovirus Vesicular stomatitis virus |
| Biosafety | 1 |
| Addtional Info | The division potential, viral susceptibilities and other properties have been thoroughly studied such that the line may be considered as an alternate for WI-38 and other standard human lung cell strains. The cells have been reported to be capable of attaining 58 population doublings before the onset of senescence. |
| Culture Medium | Dulbecco's Modified Eagle's Medium (DMEM) with 1% non-essential amino acids, 2 mM L-glutamine, 1 mM sodium pyruvate, 1.0 g/L glucose and 10% of fetal bovine serum. |
| Subculturing | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. T-75 flasks are recommended for subculturing this product. Remove and discard culture medium. Briefly rinse the cell layer with PBS without calcium and magnesium to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. NOTE: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 12 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 6th edition, published by Alan R. Liss, N.Y., 2010. |
| Subculturing Medium Renewal | Every 3 to 4 days |
| Subculturing Subcultivation Ratio | 1:2 to 1:8 |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | 22381: Nichols WW, et al. Characterization of a new human diploid cell strain, IMR-90. Science 196: 60-63, 1977. PubMed: 841339 32932: Dolganov GM, et al. Human Rad50 is physically associated with human Mre11: identification of a conserved multiprotein co |
| Depositors | Luiz Soares/ Priscila Menezes; Grupo Boticario. |
| Cellosaurus | CVCL_0347 |
