| BCRJ Code | 0273 |
| Cell Line | J774 1.6 |
| Species | Mus musculus |
| Vulgar Name | Mouse |
| Tissue | Reticulum |
| Cell Type | Macrophage-Like |
| Morphology | Macrophage |
| Disease | Sarcoma |
| Growth Properties | Adherent |
| Products | nitric oxide |
| Biosafety | 1 |
| Culture Medium | Dulbecco's Modified Eagle's Medium (DMEM) with 1% non-essential amino acids, 2 mM L-glutamine, 1.0 g/L glucose and 10% of fetal bovine serum. |
| Subculturing | Subcultures are prepared by scraping. For a 75 cm2 flask, remove all but 10 mL culture medium (adjust amount accordingly for other culture vessels). Dislodge cells from the flask substrate with a cell scraper; aspirate and add appropriate aliquots of the cell suspension into new culture vessels. |
| Subculturing Medium Renewal | Twice per week |
| Subculturing Subcultivation Ratio | 1:6 to 1:8 |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | Guido Damiani et al. J. Exp. MEn. © The Rockefeller University Press. Volume 152:808-822, October 1980. |
| Depositors | Leonardo Nimrichter - Universidade Federal Do Rio De Janeiro |
| Cellosaurus | CVCL_HA24 |
