| BCRJ Code | 0125 |
| Cell Line | Jurkat, Clone E6-1 |
| Species | Homo sapiens |
| Vulgar Name | Human |
| Tissue | Peripheral Blood |
| Cell Type | T Lymphocyte |
| Morphology | Lymphoblast |
| Disease | Acute T Cell Leukemia |
| Growth Properties | Suspension |
| Sex | Male |
| Derivation | The Jurkat cell line was established from the peripheral blood of a 14 year old boy by Schneider et al., and was originally designated JM. |
| Applications | This cell line is a suitable transfection host. |
| DNA Profile | Amelogenin: X,Y CSF1PO: 11,12 D13S317: 8,12 D16S539: 11 D5S818: 9 D7S820: 8,12 THO1: 6,9.3 TPOX: 8,10 vWA: 18 |
| Products | Interleukin 2 (IL-2), human alpha interferon |
| Biosafety | 1 |
| Addtional Info | Clone E6-1 cells produce large amounts of IL-2 after stimulation with phorbol esters and either lectins or monoclonal antibodies against the T3 antigen (both types of stimulants are needed to induce IL-2 production. |
| Culture Medium | RPMI 1640 medium with 2 mM L-glutamine, 4.5 g/L glucosen and 10% of heat-inactivated fetal bovine serum. |
| Subculturing | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 10e5 viable cells/mL. NOTE: Do not allow the cell density to exceed 3 X 10e6 cells/mL. |
| Subculturing Medium Renewal | 2 to 3 days |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | J. Exp. Med. 152: 1709-19, 1980; J. Immunol. 133: 123-128, 1984. Berninghausen O, Leippe M. Necrosis versus apoptosis as the mechanism of target cell death induced by Entamoeba histolytica. Infect. Immun. 65: 3615-3621, 1997. PubMed: 9284127 Churchill MJ, et al. The rev-responsive element negatively regulates human immunodeficiency virus type 1 env mRNA expression in primate cells. J. Virol. 70: 5786-5790, 1996. PubMed: 8709194 Kolanus W, et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cell 86: 233-242, 1996. PubMed: 8706128 |
| Depositors | Pedro Paulo Elssas, Instituto Oswaldo Cruz. |
| Cellosaurus | CVCL_0367 |
