| BCRJ Code | 0127 |
| Cell Line | K-562 LUCENA |
| Species | Homo sapiens |
| Vulgar Name | Human |
| Tissue | Hematopoietic |
| Morphology | Lymphoblast |
| Disease | Chronic Myelogenous Leukemia |
| Growth Properties | Suspension |
| Sex | Female |
| Age/Ethinicity | 53 Year / |
| Derivation | The K562-Lucena cell line was established from K562 cell line under pressure of gradual vincristine supplement in culture medium. It express the P-Glicoprotei and has a Multi Drug Resistance (MDR) phenotype. |
| DNA Profile | Amelogenin: X CSF1PO: 9,10 D13S317: 8 D16S539: 11,12 D5S818: 11,12 D7S820: 9,11 THO1: 9.3 TPOX: 8,9 vWA: 16 |
| Tumor Formation: | Yes, in nude mice Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells. |
| Products | Glicoprotein - P ( MDR-1) |
| Biosafety | 1 |
| Culture Medium | RPMI-1640 medium modified to contain 2 mM L-glutamine, 4500 mg/L glucose, 60nM vincristine and 10% of fetal bovine serum. |
| Subculturing | Cultures can be maintained by the addition or replacement of fresh medium. Start new cultures at 1 x 10e5 viable cells/mL. Subculture at 1 x 10e6 cells/mL. T-75 flasks are recommended for subculturing this product. |
| Subculturing Medium Renewal | Every 2 to 3 days |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | Ciência e Cultura, 46:63-69, 1994 Braz.J. Med. Biol. Res, 29:401-542, 1996 |
| Depositors | Vivian Mary Barral Dodd Rumjanek - Universidade Federal do Rio de Janeiro |
| Cellosaurus | CVCL_D162 |
