| BCRJ Code | 0318 |
| Cell Line | KATO III |
| Species | Homo sapiens |
| Vulgar Name | Human |
| Tissue | Stomach; Derived From Metastatic Site: Pleural Effusion And Supraclavicular And Axillary Lymph Nodes And Douglas Cul-De-Sac |
| Morphology | Spherical |
| Disease | Gastric Carcinoma |
| Growth Properties | Mixed, Adherent And Suspension |
| Sex | Male |
| Age/Ethinicity | 55 Year / Asian |
| Derivation | derived from metastatic site: pleural effusion and supraclavicular and axillary lymph nodes and Douglas cul-de-sac |
| DNA Profile | Amelogenin: X CSF1PO: 7,11 D13S317: 8,12 D16S539: 10,12 D5S818: 10,11 D7S820: 8,12 THO1: 7,9 TPOX: 11 vWA: 14,16 |
| Tumor Formation: | YES; IN CHEEK POUCHES OF ANTI THYMOCYTE SERUM TREATED HAMSTERS; NOT TUMORIGENIC IN NUDE MICE |
| Biosafety | 1 |
| Culture Medium | Iscove's Modified Dulbecco's Medium (IMDM) contains 2 mM L-glutamine, 4500 mg/L glucose and fetal bovine serum to a final concentration of 20%. |
| Subculturing | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove culture medium with floating cells to a centrifuge tube. Briefly rinse the cell layer with PBS without calcium and magnesium to remove all traces of serum, which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 10 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. To remove trypsin-EDTA solution, transfer cell suspension to the centrifuge tube with the medium and cells from step #1 and spin at approximately 125 x g for 5 to 10 minutes. Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels. 6. Place culture vessels in incubator at 37°C. NOTE: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 12 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 6th edition, published by Alan R. Liss, N.Y., 2010. |
| Subculturing Medium Renewal | Every 2 to 3 days |
| Subculturing Subcultivation Ratio | 1:2 to 1:8 is recommended |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | 2371: Sekiguchi M, et al. Establishment of cultured cell lines derived from a human gastric carcinoma. Jpn. J. Exp. Med. 48: 61-68, 1978.; Faust JB, Meeker TC. Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463, 1992. ; Rieder G, et al. Role of adherence in Interleukin-8 induction in Helicobacter pylori-associated gastritis. Infect. Immun. 65: 3622-3630, 1997. |
| Depositors | RENATO J. S OLIVEIRA (CPOM) - HOSPITAL DE CÂNCER DE BARRETOS |
| Cellosaurus | CVCL_0371 |
