| BCRJ Code | 0129 |
| Cell Line | KB 16 |
| Species | Homo sapiens |
| Vulgar Name | Human |
| Tissue | Cervix, Hela Contaminant |
| Morphology | Epithelial |
| Disease | Carcinoma, Papilloma |
| Growth Properties | Adherent |
| Sex | Male |
| Derivation | The KB 16 cell line was derived from KB cells (BCRJ 0128). It was transfected and actually contains the E1A and E1B regions of the Ad2 virus. This cell line has HeLa markers. |
| DNA Profile | Amelogenin: X CSF1PO: 9,10 D13S317: 12,13.3 D16S539: 9,10 D5S818: 11,12 D7S820: 8,12 THO1: 7 TPOX: 8,12 vWA: 16,18 |
| Products | Keratin |
| Biosafety | 2 |
| Culture Medium | Dulbecco's Modified Eagle's Medium (DMEM) with 1% non-essential amino acids, 2 mM L-glutamine, 1 mM sodium pyruvate, and 1500 mg/L sodium bicarbonate, 1.0 g/L glucose, 90%; fetal bovine serum, 10%. |
| Subculturing | Remove medium, and rinse with PBS without calcium and magnesium. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. NOTE: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 12 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 6th edition, published by Alan R. Liss, N.Y., 2010. |
| Subculturing Medium Renewal | 2 to 3 times per week |
| Subculturing Subcultivation Ratio | 1:4 to 1:10 |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | Proc. Soc. Exp. Biol. Med. 89: 362-, 1955; Virol. 46: 454-465, 1983. |
| Depositors | Jose Paulo Leite, Instituto Oswaldo Cruz, Rio de Janeiro. |
| Cellosaurus | CVCL_D680 |
