| BCRJ Code | 0132 |
| Cell Line | KG-1 |
| Species | Homo sapiens |
| Vulgar Name | Human |
| Tissue | Bone Marrow |
| Cell Type | Macrophage |
| Morphology | Myeloblast |
| Disease | Acute Myelogenous Leukemia |
| Growth Properties | Suspension |
| Sex | Male |
| Age/Ethinicity | 59 Year / Caucasian |
| Applications | This cell line is a suitable transfection host. |
| DNA Profile | Amelogenin: X,Y CSF1PO: 7 D13S317: 11,12 D16S539: 10,11 D5S818: 13 D7S820: 8,10 THO1: 7,8 TPOX: 7,9 vWA: 14,19 |
| Products | HLA DR |
| Biosafety | 1 |
| Addtional Info | KG-1 cells spontaneously differentiate to granulocyte and macrophage like cells. They show a good response to colony stimulating factor (CSF). |
| Culture Medium | Iscove's Modified Dulbecco's Medium (IMDM) contains 2 mM L-glutamine, 4500 mg/L glucose and fetal bovine serum to a final concentration of 20%. |
| Subculturing | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension. Maintain at 1.0 x 10e5 and 1.0 x 10e6 cells/mL. Population Doubling Time: 38 hrs |
| Subculturing Medium Renewal | Twice per week |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | Koeffler HP, Golde DW. Human myeloid leukemia cell lines: a review. Blood 56: 344-350, 1980. PubMed: 6996765 Koeffler HP, Golde DW. Acute myelogenous leukemia: a human cell line responsive to colony-stimulating activity. Science 200: 1153-1154, 1978. PubMed: 306682 Penrose JF, et al. Molecular cloning of the gene for human leukotriene C4 synthase. J. Biol. Chem. 271: 11356-11361, 1996. PubMed: 8626689 Hester JP et al. Principles of blood separation and component extraction in a disposable continuous-flow single-stage channel. Blood 54(1): 254-268 1979. PubMed: 444670 |
| Depositors | Universitú De Marseille, Marseille, France. |
| Cellosaurus | CVCL_0374 |
