| BCRJ Code | 0261 |
| Cell Line | KU812 |
| Species | Homo sapiens |
| Vulgar Name | Human |
| Tissue | Peripheral Blood |
| Cell Type | Basophil |
| Morphology | Myeloblast |
| Disease | Chronic Myelogenous Leukemia |
| Growth Properties | Suspension |
| Sex | Male |
| Age/Ethinicity | 38 Year / Japanese |
| Derivation | The KU812 cell line was established from the peripheral blood of a patient in blast crisis of chronic myelogenous leukemia. |
| Products | TRANSFORMING GROWTH FACTOR BETA (TGF BETA ) |
| Biosafety | 1 |
| Addtional Info | The cells contain at least one Ph1 (Philadelphia) chromosome. The cell line has some characteristics of basophilic leukocytes (Fc receptors, basophilic granules, histamine production), and are negative for lymphoid markers. The cell line has some characteristics of basophilic leukocytes (Fc receptors, basophilic granules, histamine production), and are negative for lymphoid markers. |
| Culture Medium | RPMI-1640 medium modified to contain 2 mM L-glutamine, 4500 mg/L glucose 10% of fetal bovine serum. |
| Subculturing | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 X 10e5 viable cells/mL. NOTE: Do not allow the cell concentration to exceed 3 x 10e6 cells/mL. Population Doubling Time: 20 to 30 hours |
| Subculturing Medium Renewal | Every 2 to 3 days |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | 44220: Kishi K. A new leukemia cell line with Philadelphia chromosome characterized as basophil precursors. Leuk. Res. 9: 381-390, 1985. PubMed: 3858609 44221: Okano H, et al. Hemoglobin synthesis of both adult and fetal types in a human CML cell line. J. Biochem. 104: 162-164, 1988. PubMed: 2460439 44222: Okamura S, et al. Expression of cytokine genes in hematological malignancies. Nippon Ketsueki Gakkai Zasshi 52: 1423-1432, 1989. PubMed: 2698048 61145: Blom T, et al. Phenotypic characterization of KU812, a cell line identified as an immature human basophilic leukocyte. Eur. J. Immunol. 22: 2025-2032, 1992. PubMed: 1639103 |
| Depositors | Fernando Ferreira Costa, Hemocentro. |
| Cellosaurus | CVCL_0379 |
