| BCRJ Code | 0140 |
| Cell Line | L5178Y TK+/- clone (3.7.2C) [TK+/- (clone 3.7.2C)] |
| Species | Mus musculus |
| Vulgar Name | Mouse; Dba/2 |
| Tissue | Thymus |
| Morphology | Lymphoblast |
| Disease | Lymphoma |
| Growth Properties | Suspension |
| Derivation | The transplantable murine leukemia L5178 was derived from a thymic tumor induced in a DBA/2 mouse by methylcolanthrene. The tumor ws adapted in culture in 1958, and established as cell line growing in suspension, designated L5178y. |
| Applications | The cell line can be used in genotoxicity tests including gene mutation assays at the tk and other loci and micronucleus induction. |
| Tumor Formation: | in DBA/2 mice |
| Biosafety | 1 |
| Culture Medium | Dulbecco's modified Eagle's medium with 4 mM L-glutamin, 4.5 g/L glucose, 0.1% Pluronic and 10% fetal bovine serum. |
| Subculturing | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension. Maintain cultures from 1x10e5 to 1x10e6 cells/mL. |
| Subculturing Medium Renewal | Every 3 to 4 days |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | Sawyer JR, Binz RL, Wang J, Moore MM. 2006 Multicolor spectral karyotyping of the L5178Y Tk+/- -3.7.2C mouse lymphoma cell line. Environ Mol Mutagen.47(2):127-31.PMID: 16247762 Chem. Mutagens 3: pp. 79-103, 1973. |
| Depositors | Cristina Massoco; Ciallyx |
| Cellosaurus | CVCL_6665 |
