| BCRJ Code | 0149 |
| Cell Line | LNCaP clone FGC |
| Species | Homo sapiens |
| Vulgar Name | Human |
| Tissue | Prostate; Derived From Metastatic Site: Left Supraclavicular Lymph Node |
| Morphology | Epithelial |
| Disease | Carcinoma |
| Growth Properties | Adherent, Single Cells And Loosely Attached Clusters |
| Sex | Male |
| Age/Ethinicity | 50 Year / Caucasian |
| Derivation | LNCaP clone FGC was isolated in 1977 by J.S. Horoszewicz, et al., from a needle aspiration biopsy of the left supraclavicular lymph node of a 50-year-old Caucasian male (blood type B+) with confirmed diagnosis of metastatic prostate carcinoma. |
| Applications | This cell line is suitable as a transfection host. |
| DNA Profile | Amelogenin: X,Y CSF1PO: 10,11 D13S317: 10,12 D16S539: 11 D5S818: 11,12 D7S820: 9.1,10.3 THO1: 9 TPOX: 8,9 vWA: 16,18 |
| Tumor Formation: | Yes, in soft agar Yes, the cells are tumorigenic in nude mice |
| Products | human prostatic acid phosphatase; prostate specific antigen |
| Biosafety | 1 |
| Addtional Info | These cells are responsive to 5-alpha-dihydrotestosterone (growth modulation and acid phosphatase production). The cells do not produce a uniform monolayer, but grow in clusters which should be broken apart by repeated pipetting when subcultures are prepared. They attach only lightly to the substrate, do not become confluent and rapidly acidify the medium. Growth is very slow. The cells should be allowed to incubate undisturbed for the first 48 hours after subculture. When flask cultures are shipped, the majority of the cells become detached from the flask and float in the medium. Upon receipt, incubate the flask (in the usual position for monolayer cultures) for 24 to 48 hours to allow the cells to re-attach. The medium can then be removed and replaced with fresh medium. If desired, the contents of the flask can be collected, centrifuged at 300 X g for 15 minutes, resuspended in 10 mL of medium and dispensed into a single flask. |
| Culture Medium | RPMI-1640 medium modified to contain 2 mM L-glutamine, 1 mM sodium pyruvate, 4500 mg/L glucose and 20% of fetal bovine serum. |
| Subculturing | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. T-75 flasks are recommended for subculturing this product. Remove and discard culture medium. Briefly rinse the cell layer with with PBS without calcium and magnesium to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Maintain cultures at a cell concentration between 1 X 10e4 and 2 X 10e5 cells/cm2. Incubate cultures at 37°C. Population Doubling Time: about 34 hours NOTE: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 12 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 6th edition, published by Alan R. Liss, N.Y., 2010. |
| Subculturing Medium Renewal | Twice per week |
| Subculturing Subcultivation Ratio | 1:3 to 1:6 |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | Models for prostate cancer. 37New York: Liss; 1980; Gibas Z, et al. A high-resolution study of chromosome changes in a human prostatic carcinoma cell line (LNCaP). Cancer Genet. Cytogenet. 11: 399-404, 1984. PubMed: 6584201 |
| Depositors | MARIA DE FATIMA MAGALHAES LAZARI; INFAR- UNIFESP |
| ATCC | CRL-1740 |
| Cellosaurus | CVCL_1379 |
