BCRJ Code | 0175 |
Cell Line | M-NFS-60 |
Species | Mus musculus |
Vulgar Name | Mouse |
Cell Type | Virus Induced |
Morphology | Lymphoblast |
Disease | Myelogenous Leukemia |
Growth Properties | Suspension |
Derivation | The M-NFS-60 cell line was derived from a myelogenous leukemia induced with the Cas-Br-MuLV wild mouse ecotropic retrovirus. |
Applications | This cell line is a suitable transfection host. |
Biosafety | 1 |
Addtional Info | The cells are responsive to both interleukin 3 (interleukin-3, IL-3) and macrophage colony stimulating factor (M-CSF). The cells contain a truncated c-myb proto - oncogene caused by integration of a retrovirus. |
Culture Medium | RPMI-1640 medium modified to contain 2 mM L-glutamine, 4500 mg/L glucose, 0.05 mM 2-mercaptoethanol, 62 ng/ml human recombinant macrophage colony stimulating factor (M-CSF) and 10% of fetal bovine serum. |
Subculturing | Cultures can be maintained by addition or replacement of fresh medium. Subculture every two days at 2.5 X 10e4 viable cells/mL. |
Subculturing Medium Renewal | Add fresh medium at the time of subculture |
Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
References | 22842: Nakoinz I, et al. Differentiation of the IL-3-dependent NFS-60 cell line and adaption to growth in macrophage colony-stimulating factor. J. Immunol. 145: 860-864, 1990. PubMed: 2142710 23295: Weinstein Y, et al. Truncation of the c-myb gene by a re |
Depositors | Cristina Massoco; Ciallyx |
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