| BCRJ Code | 0432 |
| Cell Line | MDA-MB-361 |
| Species | Homo sapiens |
| Vulgar Name | Human |
| Tissue | Breast; Mammary gland (brain metastasis) |
| Cell Type | Epithelial |
| Morphology | Epithelial |
| Disease | Adenocarcinoma |
| Growth Properties | Loosely Adherent |
| Sex | Female |
| Age/Ethinicity | 40 Year / White |
| Applications | 3D cell culture; Neuroscience |
| Products | Genes expressed: wnt7h+; Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; Me-2, 1; PGM1, 1; PGM3, 1-2 |
| Biosafety | 1 |
| Addtional Info | This line differs from others of the series in karyology and in that it was isolated from a brain metastasis. The cell line is aneuploid human female, with chromosome counts in the hyperdiploid range. Normal chromosomes N11 and N17 are absent, chromosomes N1, N20, and N21 are weakly represented, and chromosomes N2, N8, N9, and N15 are single. The remainder of chromosomes are often paired. Eighteen marker chromosomes are found, of which 10 are consistently present. Some of these markers are found to be quite comparable to those described by K.L. Satya-Prakash, et al., in their report on this cell line. |
| Culture Medium | Leibovitz's L-15 Medium and fetal bovine serum to a final concentration of 20%. |
| Subculturing | Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. |
| Subculturing Medium Renewal | 2 to 3 times per week |
| Subculturing Subcultivation Ratio | 1:2 to 1:6. Split sub-confluent cultures (70-80%) or seeding at 3-6x10,000 cells/cm². |
| Culture Conditions | Atmosphere: air, 100% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | Brinkley BR, et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980. PubMed: 7000337 Cruciger Q, et al. Morphological, biochemical and chromosomal characterization of breast tumor lines from pleural effusions. In Vitro 12: 331, 1976. Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779 Cailleau R, et al. Breast tumor cell lines from pleural effusions. J. Natl. Cancer Inst. 53: 661-674, 1974. PubMed: 4412247 Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 |
| Depositors | Banco de Células do Rio de Janeiro |
| Cellosaurus | CVCL_0620 |
