| BCRJ Code | 0262 |
| Cell Line | MEG-01 |
| Species | Homo sapiens |
| Vulgar Name | Human |
| Tissue | Bone Marrow |
| Cell Type | Megakaryoblast |
| Morphology | Lymphoblast |
| Disease | Chronic Myelogenous Leukemia, |
| Growth Properties | Mixed, Adherent And Suspension |
| Sex | Male |
| Age/Ethinicity | 55 Year / |
| Derivation | The MEG-01 cell line was derived in 1983 at the Nagoya University School of Medicine, Nagoya, Japan from bone marrow cells taken from a patient in megakaryoblastic crisis of CML. |
| Applications | This cell line is a suitable transfection host. |
| DNA Profile | Amelogenin: X,Y CSF1PO: 10 D13S317: 8 D16S539: 9 D5S818: 13 D7S820: 11 THO1: 7 TPOX: 8,11 vWA: 16 |
| Biosafety | 1 |
| Culture Medium | RPMI-1640 medium modified to contain 2 mM L-glutamine, 4500 mg/L glucose and 10% of fetal bovine serum. |
| Subculturing | Subcultures are prepared by scraping the adherent cells into the medium. From the resulting suspension dilute cells to a concentration 1-2 X 10e5 cells/mL into fresh medium in a new flasks. Keep culture below approximate density of 10e6 cells/mL. Subcultures are prepared by scraping the adherent cells into the medium, and diluting the resulting suspension into fresh medium in new flasks. |
| Subculturing Medium Renewal | 2 to 3 times per week |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | 23348: Ogura M, et al. Establishment of a novel human megakaryoblastic leukemia cell line, MEG- 01, with positive Philadelphia chromosome. Blood 66: 1384-1392, 1985. PubMed: 2998511 |
| Depositors | Andrea Cheble de Oliveira, Instituto de Bioquíimica Médica, RJ. |
| Cellosaurus | CVCL_0425 |
