| BCRJ Code | 0293 |
| Cell Line | MIN-6 |
| Species | Mus musculus |
| Vulgar Name | Mouse |
| Tissue | Pancreas |
| Disease | Tumor |
| Growth Properties | Adherent |
| Products | MIN6 cells excreted insulin, glucagon, somatostatin and ghrelin. |
| Biosafety | 2 |
| Addtional Info | MIN6 cells excreted insulin, glucagon, somatostatin and ghrelin. |
| Culture Medium | Dulbecco's Modified Eagle's Medium (DMEM) low glucose with 2 mM L-glutamine and 10% fetal bovine serum. |
| Subculturing | Subculture when 80% confluent or less. Remove and discard culture medium. Briefly rinse the cell layer with PBS without calcium and magnesium to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension into new culture vessels. Incubate cultures at 37°C. NOTE: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 12 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 6th edition, published by Alan R. Liss, N.Y., 2010. |
| Subculturing Medium Renewal | 2 to 3 times per week |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | Garay-Malpartida et al.: Toll-like receptor 4 (TLR4) expression in human and murine pancreatic beta-cells affects cell viability and insulin homeostasis. BMC Immunology 2011 12:18; Helen Roderigo-Milne et al: Differential expression of insulin genes 1 and 2 in MIN6 cells and pseudoislets. Biochemical and Biophysical Research Communications 296 (2002) 589–595. Nakashima k, MIN6 is not a pure beta cell line but a mixed cell line with other pancreatic endocrine hormones. Endocr J. 2009;56(1):45-53, 2008. PubMed: 18845907 |
| Depositors | Fernanda Paladino; Instituto Israelita De Ensino E Pesquisa |
| Cellosaurus | CVCL_0431 |
