| BCRJ Code | 0176 |
| Cell Line | MOLT-4 |
| Species | Homo sapiens |
| Vulgar Name | Human |
| Tissue | Peripheral Blood |
| Cell Type | T Lymphoblast |
| Morphology | Lymphoblast |
| Disease | Acute Lymphoblastic Leukemia |
| Growth Properties | Suspension |
| Sex | Male |
| Age/Ethinicity | 19 Year / |
| Derivation | A suspension culture derived from the peripheral blood of a 19 year old male with acute lymphoblastic leukaemia in relapse. A stable T-cell leukaemia that forms rosettes with sheep erythrocytes. |
| Applications | This cell line is a suitable transfection host. |
| DNA Profile | Amelogenin: X,Y CSF1PO: 11, 12, 13 D13S317: 12, 13 D16S539: 11, 14 D5S818: 12 D7S820: 8, 10, 11 THO1: 6, 8 TPOX: 8 vWA: 17, 18 |
| Tumor Formation: | Yes, in untreated nude mice, anti lymphocyte serum treated mice and X-irradiated mice |
| Products | high levels of terminal deoxynucleotidyl transferase (TdT) are produced |
| Biosafety | 1 |
| Culture Medium | RPMI-1640 medium modified to contain 2 mM L-glutamine, 4500 mg/L glucose and 10% of fetal bovine serum. |
| Subculturing | Cultures can be maintained by addition or replacement of fresh medium. Start new cultures at 4 X 10e5 cells/mL and subculture before the cell density reaches 2 X 10e6 cells/mL. |
| Subculturing Medium Renewal | Every 2 to 3 days |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | Minowada J, et al. Rosette-forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus-derived lymphocytes. J. Natl. Cancer Inst. 49: 891-895, 1972. PubMed: 4567231 Ohsugi Y, et al. Tumorigenicity of human malignant lymphoblasts: comparative study with unmanipulated nude mice, antilymphocyte serum-treated nude mice, and X- irradiated nude mice. J. Natl. Cancer Inst. 65: 715-718, 1980. PubMed: 6932523 Mertelsmann R, et al. T-cell growth factor (interleukin 2) and terminal transferase activity in human leukemias and lymphoblastic cell lines. Blut 43: 99-103, 1981. PubMed: 6942897 Rodrigues NR, et al. p53 mutations in colorectal cancer. Proc. Natl. Acad. Sci. USA 87: 7555-7559, 1990. PubMed: 1699228 Sandstrom PA, Buttke TM. Autocrine production of extracellular catalase prevents apoptosis of the human CEM T-cell line in serum-free medium. Proc. Natl. Acad. Sci. USA 90: 4708-4712, 1993. PubMed: 8506323 |
| Depositors | Eliana Saul Abdelhay; Universidade Federal Do Rio De Janeiro |
| ATCC | CRL-1582 |
| Cellosaurus | CVCL_0013 |
