| BCRJ Code | 0373 |
| Cell Line | MONO-MAC-6 |
| Species | Homo sapiens |
| Vulgar Name | Human |
| Morphology | Single, round/multiformed cells or small clusters of cells in suspension, sometimes loosely adherent; 1-5% giant cells. |
| Disease | Acute Monocytic Leukemia |
| Growth Properties | suspension, sometimes loosely adherent |
| Sex | Male |
| Age/Ethinicity | 64 Year / |
| Derivation | Established from the peripheral blood of a 64-year-old man with relapsed acute monocytic leukemia (AML FAB M5) in 1985 following myeloid metaplasia. |
| Biosafety | 1 |
| Culture Medium | RPMI 1640 with 2 mM L-glutamine, 10 µg/mL human insulin and 10% of fetal bovine serum. |
| Subculturing | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 0,3 X 106 viable cells/mL. Maintain cell density between 0,3 X 106 and 1.0 X 106 viable cells/mL. NOTE: Do not allow the cell concentration to exceed 3 x 10e6 cells/mL. |
| Subculturing Subcultivation Ratio | 1:3 a 1:5 |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | Drexler, H. G., Quentmeier, H., MacLeod, R. A. (2004). Malignant hematopoietic cell lines: in vitro models for the study of MLL gene alterations. Leukemia : official journal of the Leukemia Society of America, Leukemia Research Fund, U.K 18 ( 2 ): 227-232 . http://www.ncbi.nlm.nih.gov/pubmed/14671638 Ziegler-Heitbrock, H. W., Thiel, E., Futterer, A., Herzog, V., Wirtz, A., Riethmuller, G. (1988). Establishment of a human cell line (Mono Mac 6) with characteristics of mature monocytes. International journal of cancer.Journal international du cancer 41 ( 3 ): 456-461 . http://www.ncbi.nlm.nih.gov/pubmed/3162233. Taktak, Y. S., Selkirk, S., Bristow, A. F., Carpenter, A., Ball, C., Rafferty, B., Poole, S. (1991). Assay of pyrogens by interleukin-6 release from monocytic cell lines. J Pharm.Pharmacol 43 ( 8 ): 578-582 . http://www.ncbi.nlm.nih.gov/pubmed/1681074. Hoffmann, S., Peterbauer, A., Schindler, S., Fennrich, S., Poole, S., Mistry, Y., Montag-Lessing, T., Spreitzer, I., Loschner, B., van, A. M., Bos, R., Gommer, M., Nibbeling, R., Werner-Felmayer, G., Loitzl, P., Jungi, T., Brcic, M., Brugger, P., Frey, E., Bowe, G., Casado, J., Coecke, S., de, L. J., Mogster, B., Naess, L. M., Aaberge, I. S., Wendel, A., Hartung, T. (2005). International validation of novel pyrogen tests based on human monocytoid cells. J Immunol.Methods 298 ( 1-2 ): 161-173 . http://www.ncbi.nlm.nih.gov/pubmed/15847806 (2012). The Monocytoid Cell Line Mono Mac 6 (MM6)/IL-6 In Vitro Pyrogen Test. ICCVAM Test Method Evaluation Report: Appendix C5 http://iccvam.niehs.nih.gov/docs/pyrogen/TMER/Appx-C5.pdf. |
| Depositors | Banco de Células do Rio de Janeiro |
| Cellosaurus | CVCL_1426 |
