| BCRJ Code | 0181 |
| Cell Line | NAMALWA |
| Species | Homo sapiens |
| Vulgar Name | Human |
| Tissue | Blood |
| Cell Type | B Lymphocyte |
| Morphology | Lymphoblast |
| Disease | Burkitt´S Lymphoma |
| Growth Properties | Suspension |
| Sex | Female |
| Age/Ethinicity | 3 Year / |
| Applications | This cell line is a suitable transfection host. |
| DNA Profile | Amelogenin: X CSF1PO: 10,11 D13S317: 11,12 D16S539: 9 D5S818: 12,13 D7S820: 11 THO1: 7,9.3 TPOX: 6,11 vWA: 14 |
| Products | immunoglobulin |
| Biosafety | 2 |
| Addtional Info | Secretes small amounts of an IgM monoclonal antibody of unknown specificity. It has been used for commercial production of human interferon. The cells contain the Epstein-Barr virus (EBV) genome. |
| Culture Medium | RPMI-1640 medium modified to contain 2 mM L-glutamine, 4500 mg/L glucose and 10% of fetal bovine serum. |
| Subculturing | Cultures can be maintained by addition or replacement of fresh medium. Cultures should be started at 5 x 10e5 viable cells/mL and subcultured at 2 x 10e6 cells/mL. |
| Subculturing Medium Renewal | Every 2 to 3 days |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | Klein F, et al. Large-scale production and concentration of human lymphoid interferon. Antimicrob. Agents Chemother. 15: 420-427, 1979. PubMed: 464569 Benjamin D, et al. Immunoglobulin secretion by cell lines derived from African and American undifferentiated lymphomas of Burkitt's and non-Burkitt's type. J. Immunol. 129: 1336-1342, 1982. PubMed: 6286763 Klein G, et al. Sensitivity of Epstein-Barr virus (EBV) producer and non-producer human lymphoblastoid cell lines to superinfection with EB-virus. Int. J. Cancer 10: 44-57, 1972. PubMed: 4122458 Nyormoi O, et al. Sensitivity to EBV superinfection and IUdR inducibility of hybrid cells formed between a sensitive and a relatively resistant Burkitt lymphoma cell line. Int. J. Cancer 12: 396-408, 1973. PubMed: 4365093 |
| Depositors | Universitú De Marseille, Marseille, France. |
| Cellosaurus | CVCL_0067 |
