| BCRJ Code | 0187 |
| Cell Line | NCI-H292 [H292] |
| Species | Homo sapiens |
| Vulgar Name | Human |
| Tissue | Lung |
| Cell Type | Epithelial |
| Morphology | Epithelial |
| Disease | Mucoepidermoid Pulmonary Carcinoma |
| Growth Properties | Adherent |
| Sex | Female |
| Age/Ethinicity | 32 Year / |
| Derivation | Derived from a cervical node metastasis of a pulmonary mucoepidermoid carcinoma from a 32 year old female. |
| Applications | The line has been selected as a prototype for transfecting human subgenomic fragments into human cells for studying the role of HBV and its individual genes in the pathogenesis of viral hepatitis and liver cancer. |
| DNA Profile | Amelogenin: X CSF1PO: 10 D13S317: 11,12 D16S539: 9,13 D5S818: 13 D7S820: 10 THO1: 8 TPOX: 8,11 vWA: 16,17 |
| Virus Succeptility: | HEPATITIS B VIRUS |
| Tumor Formation: | Yes, in nude mice; tumors histologically resemble the original biopsy specimen and retain mucoepidermoid features |
| Products | keratin; vimentin |
| Biosafety | 1 |
| Addtional Info | The cells retain their mucoepidermoid characteristics in culture as determined by their ultrastructure and expression of multiple markers of squamous differentiation. The cells support the growth of hepatitis B virus and are negative for L-DOPA decarboxylase. The cells stain positive for keratin and vimentin and are mucicarmine positive but are negative for neurofilament triplet protein. |
| Culture Medium | RPMI-1640 medium modified to contain 2 mM L-glutamine, 4500 mg/L glucose and 10% of fetal bovine serum. |
| Subculturing | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with PBS without calcium and magnesium to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Population Doubling Time 48 hrs NOTE: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 12 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 6th edition, published by Alan R. Liss, N.Y., 2010. |
| Subculturing Medium Renewal | Every 2 to 3 days |
| Subculturing Subcultivation Ratio | 1:3 to 1:8 |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | 1605: Banks-Schlegel SP, et al. Intermediate filament and cross-linked envelope expression in human lung tumor cell lines. Cancer Res. 45: 1187-1197, 1985. PubMed: 2578876 22946: Yoakum GH, et al. High-frequency transfection and cytopathology of the hepat |
| Depositors | JERSON LIMA DA SILVA, Universidade Federal do Rio de Janeiro |
| ATCC | CRL-1848 |
| Cellosaurus | CVCL_0455 |
