| BCRJ Code | 0416 |
| Cell Line | NCI-H460 [H460] |
| Species | Homo sapiens |
| Vulgar Name | Human |
| Tissue | Lung; Pleural effusion |
| Cell Type | Epithelial |
| Morphology | Epithelial |
| Disease | Carcinoma |
| Growth Properties | Adherent |
| Sex | Male |
| Derivation | The NCI-H460 cell line was derived by A.F. Gazdar and associates in 1982 from the pleural fluid of a patient with large cell cancer of the lung. |
| Applications | NCI-H460 [H460] cells were isolated in 1982 from the pleural fluid of a male patient with large cell lung cancer. Use these cells in your cancer and toxicology research. |
| DNA Profile | "D3S1358: 15,18 TH01: 9.3 D21S11: 30 D18S51: 13,15 Penta_E: 5 D5S818: 9,10 D13S317: 13 D7S820: 9,12 D16S539: 9 CSF1PO: 11,12 Penta_D: 11,13 Amelogenin: X,Y vWA: 17 D8S1179: 12 TPOX: 8 FGA: 21,23 D19S433: 14 D2S1338: 17,25" |
| Tumor Formation: | Yes; Yes, in nude mice |
| Products | "Isoenzymes: AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1-2 Me-2, 1 PGM1, 1 PGM3, 1" |
| Biosafety | 1 |
| Addtional Info | The cells express easily detectable p53 mRNA at levels comparable to normal lung tissue, and exhibit no gross structural DNA abnormalities. The cells stain positively for keratin and vimentin but are negative for neurofilament triplet protein. |
| Culture Medium | RPMI 1640 with 2.0 mM L-glutamine adjusted to contain 4.5 g/L glucose, 1.0 mM sodium pyruvate and 10% of fetal bovine serum. |
| Subculturing | "Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks." |
| Subculturing Medium Renewal | Twice a week |
| Subculturing Subcultivation Ratio | 1:3 to 1:8 is recommended |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | "Banks-Schlegel SP, et al. Intermediate filament and cross-linked envelope expression in human lung tumor cell lines. Cancer Res. 45: 1187-1197, 1985. PubMed: 2578876 Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494 Brower M, et al. Growth of cell lines and clinical specimens of human non-small cell lung cancer in a serum-free defined medium. Cancer Res. 46: 798-806, 1986. PubMed: 3940644 Geiger T, et al. Antitumor activity of a PKC-alpha antisense oligonucleotide in combination with standard chemotherapeutic agents against various human tumors transplanted into nude mice. Anticancer Drug Des. 13: 35-45, 1998. PubMed: 9474241" |
| Depositors | Carlos Augusto Gomes Soares - UFRJ |
| Cellosaurus | CVCL_0459 |
