| BCRJ Code | 0303 |
| Cell Line | NTERA-2 cl.D1 [NT2/D1] |
| Species | Homo sapiens |
| Vulgar Name | Human |
| Tissue | Testis; Derived From Metastatic Site: Lung |
| Morphology | Epithelial-Like,Differentiation Changes Phenotype |
| Disease | Malignant Pluripotent Embryonal Carcinoma |
| Growth Properties | Adherent |
| Sex | Male |
| Age/Ethinicity | 22 Year / Caucasian |
| Derivation | The parental NTERA-2 lines was established in 1980 from a nude mouse xenograft of the Tera-2 cell line. The NTERA-2 cl.D1 cell line is a pluripotent human testicular embryonal carcinoma cell line derived by cloning the NTERA-2 cell line. |
| Applications | This cell line is a suitable transfection host. |
| DNA Profile | Amelogenin: X,Y CSF1PO: 10,12 D13S317: 13 D16S539: 11,12,13 D5S818: 9,12 D7S820: 10,12 THO1: 9.3 TPOX: 8 vWA: 18,19 |
| Virus Resistance: | UNTREATED CELLS: human cytomegalovirus (HCMV); human immunodeficiency virus (HIV-1, HTLV-III) |
| Biosafety | 1 |
| Addtional Info | This clone differentiates along neuroectodermal lineages after exposure to retinoic acid (RA) or hexamethylene bisacetamide (HMBA). The RA induced differentiation is characterized by glycolipid changes, appearance of neurons, and induction of homeobox (HOX) gene clusters. The cells exhibit high expression of N-myc oncogene activity. To induce differentiation, the cells should be trypsinized and seeded at a density 1 X 10 exp6 cells per 75 sq. cm. in medium containing 0.01 mM trans-retinoic acid. Stock solutions of trans-retinoic acid (10 mM, dissolved in DMSO) should be stored frozen (preferably under a nitrogen atmosphere). |
| Culture Medium | Dulbecco's Modified Eagle's Medium (DMEM) modified to contain 2 mM L-glutamine, 4500 mg/L glucose and 10% of fetal bovine serum. |
| Subculturing | Subcultures are prepared by scraping. Cells from confluent cultures (approximately 20 million cells per 75 cm2) are dislodged from the flask surface, aspirated and dispensed into new flasks. Cultures should be maintained at high density. Seed new flasks at a density of at least 5 X 10e6 viable cells per 75 cm2 flask. |
| Subculturing Medium Renewal | Every 2 to 3 days |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | Andrews PW. Human teratocarcinomas. Biochim. Biophys. Acta 948: 17-36, 1988. PubMed: 3293662 Mavilio F, et al. Activation of four homeobox gene clusters in human embryonal carcinoma cells induced to differentiate by retinoic acid. Differentiation 37: 73-79, 1988. PubMed: 2898410 Fenderson BA, et al. Glycolipid core structure switching from globo- to lacto- and ganglio- series during retinoic acid-induced differentiation of TERA-2-derived human embryonal carcinoma cells. Dev. Biol. 122: 21-34, 1987. PubMed: 3297853 Andrews PW, et al. Pluripotent embryonal carcinoma clones derived from the human teratocarcinoma cell line Tera-2. Differentiation in vivo and in vitro. Lab. Invest. 50: 147-162, 1984. PubMed: 6694356 Andrews PW, et al. Different patterns of glycolipid antigens are expressed following differentiation of TERA-2 human embryonal carcinoma cells induced by retinoic acid, hexamethylene bisacetamide (HMBA) or bromodeoxyuridine (BUdR). Differentiation 43: 131-138, 1990. PubMed: 2373286 Gonczol E, et al. Cytomegalovirus replicates in differentiated but not in undifferentiated human embryonal carcinoma cells. Science 224: 159-161, 1984. PubMed: 6322309 Andrews PW. Retinoic acid induces neuronal differentiation of a cloned human embryonal carcinoma cell line in vitro. Dev. Biol. 103: 285-293, 1984. PubMed: 6144603 Hirka G, et al. Differentiation of human embryonal carcinoma cells induces human immunodeficiency virus permissiveness which is stimulated by human cytomegalovirus coinfection. J. Virol. 65: 2732-2735, 1991. PubMed: 1850047 Dewji NN, Singer SJ. Cell surface expression of the Alzheimer disease-related presenilin proteins. Proc. Natl. Acad. Sci. USA 94: 9926-9931, 1997. PubMed: 9275228 Baldassarre G, et al. Transfection with a CRIPTO anti-sense plasmid suppresses endogenous CRIPTO expression and inhibits transformation in a human embryonal carcinoma cell line. Int. J. Cancer 66: 538-543, 1996. PubMed: 8635871 |
| Depositors | CATARINA SEGRETI PORTO - INFAR |
| Cellosaurus | CVCL_3407 |
