| BCRJ Code | 0383 |
| Cell Line | OCI-AML2 |
| Species | Homo sapiens |
| Vulgar Name | Human |
| Tissue | Peripheral Blood |
| Morphology | Single round to oval cells |
| Disease | Leukemia, acute myeloid |
| Growth Properties | Suspension |
| Sex | Male |
| Biosafety | 1 |
| Addtional Info | Retinoic acid receptor gene expression. |
| Culture Medium | Alpha-MEM with 2mM L-glutamine and 10% of fetal bovine serum. |
| Subculturing | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension. Maintain cell density between 0,3 X 106 and 1.0 X 106 viable cells/mL. |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | Leukemia 1989;3:264-269 - PMID: 2538684 |
| Depositors | Fábio Pires - Hospital Israelita Albert Einstein |
| Cellosaurus | CVCL_1619 |
