| BCRJ Code | 0198 |
| Cell Line | P388 |
| Species | Mus musculus |
| Vulgar Name | Mouse; Dba/2 |
| Cell Type | Monocyte |
| Morphology | Macrophage |
| Disease | Lymphoid Neoplasm |
| Growth Properties | Adherent |
| Derivation | Originating in a DBA/2 mouse as lymphoid neoplasm methylcholantrene-induced, P388 cells was converted to ascit form in the first mouse transfer. |
| Biosafety | 1 |
| Addtional Info | The in vitro derived line as obtained from 49 consecutive mouse passages. These cells actively phagoc |
| Culture Medium | RPMI 1640 with 2.0 mM L-glutamine, 4.5 g/L glucose and 10% of fetal bovine serum. |
| Subculturing | Subcultures are prepared by scraping. Remove old medium, add fresh dislodge the cells and dispense into new flasks. NOTE: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 12 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 6th edition, published by Alan R. Liss, N.Y., 2010. |
| Subculturing Medium Renewal | 3 times per week |
| Subculturing Subcultivation Ratio | 1:4 to 1:8 |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | Amer. J. Pathol. 3: 33, 1957. |
| Depositors | Dr. Elayse Maria Hachich, Campinas, São Paulo. |
| Cellosaurus | CVCL_7222 |
