| BCRJ Code | 0274 |
| Cell Line | PLC/PRF/5 |
| Species | Homo sapiens |
| Vulgar Name | Human |
| Tissue | Liver |
| Cell Type | Alexander Cells |
| Morphology | Epithelial |
| Disease | Hepatoma |
| Growth Properties | Adherent |
| DNA Profile | Amelogenin: X CSF1PO: 10 D13S317: 11,12 D16S539: 13 D5S818: 12 D7S820: 9,11 THO1: 8 TPOX: 8 vWA: 15,16 |
| Products | hepatitis virus B surface antigen (HBsAg) |
| Biosafety | 2 |
| Addtional Info | The cells contain the neu transforming gene which codes for a 185000 dalton antigen designated p185. |
| Culture Medium | Dulbecco's Modified Eagle's Medium (DMEM) with 2 mM L-glutamine, 1.0 g/L glucose and 10% of fetal bovine serum. |
| Subculturing | Remove medium, and rinse with PBS without calcium and magnesium. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. NOTE: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 12 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 6th edition, published by Alan R. Liss, N.Y., 2010. |
| Subculturing Medium Renewal | Twice per week |
| Subculturing Subcultivation Ratio | 1:2 to 1:4 is recommended |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | Monjardino J, Crawford E. Polypeptide profile of HBSAg excreted by a human hepatoma cell line. Virology 96: 652-655, 1979. |
| Depositors | Debora Regina Lopes |
| Cellosaurus | CVCL_0485 |
