| BCRJ Code | 0214 |
| Cell Line | RT4 |
| Species | Homo sapiens |
| Vulgar Name | Human |
| Tissue | Urinary Bladder |
| Morphology | Epithelial |
| Disease | Transitional Cell Papilloma |
| Growth Properties | Adherent |
| Sex | Male |
| Age/Ethinicity | 63 Year / Caucasian |
| Applications | This cell line is suitable as a transfection host. |
| DNA Profile | Amelogenin: X,Y CSF1PO: 10,12 D13S317: 8 D16S539: 9 D5S818: 11,12 D7S820: 9,12 THO1: 9,9.3 TPOX: 8,11 vWA: 14,17 |
| Tumor Formation: | Yes, in cheek pouch of steroid-treated hamsters |
| Products | Antigen expression: HLA A25(10), A3, B12, Cw3; Blood Type O |
| Biosafety | 1 |
| Culture Medium | McCoy's 5A Medium is modified to contain 2 mM L-glutamine and fetal bovine serum to a final concentration of 10%. |
| Subculturing | Remove medium, and rinse with PBS without calcium and magnesium. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. NOTE: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 12 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 6th edition, published by Alan R. Liss, N.Y., 2010. |
| Subculturing Medium Renewal | Every 2 to 3 days |
| Subculturing Subcultivation Ratio | 1:2 to 1:4 is recommended |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | O'Toole C . Human bladder cancer lines: HLA Class I and Class II antigen expression and susceptibility to cytostatic and cytotoxic effects in vitro. In: Webber, M.M.; Sekely, L.I., eds., editor. In vitro models for cancer research. vol. IV: Boca Raton, FL |
| Depositors | Ana Maria Oliveira Battastini - UFRGS |
| ATCC | HTB-2 |
| Cellosaurus | CVCL_0036 |
