| BCRJ Code | 0217 |
| Cell Line | Saos-2 |
| Species | Homo sapiens |
| Vulgar Name | Human |
| Tissue | Bone |
| Morphology | Epithelial |
| Disease | Osteosarcoma |
| Growth Properties | Adherent |
| Sex | Female |
| Age/Ethinicity | 11 Year / Caucasian |
| Applications | This cell line is suitable as a transfection host. |
| DNA Profile | Amelogenin: X CSF1PO: 10 D13S317: 12,13 D16S539: 12,13 D5S818: 12 D7S820: 8,10 THO1: 6,9 TPOX: 8 vWA: 18 |
| Tumor Formation: | No, The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium. |
| Biosafety | 1 |
| Addtional Info | The patient was treated with RTG, methotrexate, adriamycin, vincristine, cytoxan, and aramycin-C. |
| Culture Medium | McCoy's 5A Medium is modified to contain 2 mM L-glutamine and fetal bovine serum to a final concentration of 10%. |
| Subculturing | Remove medium, and rinse with PBS without calcium and magnesium. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. NOTE: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 12 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 6th edition, published by Alan R. Liss, N.Y., 2010. |
| Subculturing Medium Renewal | 1 to 2 times per week |
| Subculturing Subcultivation Ratio | 1:2 to 1:4 |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | Banerjee C , et al. An AML-1 consensus sequence binds an osteoblast-specific complex and transcriptionally activates the osteoclacin gene. Proc. Natl. Acad. Sci. USA 93: 4968-4973, 1996. Fogh J, editor. Human tumor cells in vitro. 93: New York: Plenum Pr |
| Depositors | Marcos Farina de Souza - Universidade Federal do Rio de Janeiro. |
| Cellosaurus | CVCL_0548 |
