| BCRJ Code | 0195 |
| Cell Line | SCC-4 |
| Species | Homo sapiens |
| Vulgar Name | Human |
| Tissue | Tongue |
| Morphology | Epithelial-Like |
| Disease | Squamous Cell Carcinoma |
| Growth Properties | Adherent |
| Sex | Male |
| Age/Ethinicity | 55 Year / |
| DNA Profile | Amelogenin: X,Y CSF1PO: 11 D13S317: 11,13 D16S539: 12 D5S818: 13 D7S820: 9,11 THO1: 9.3 TPOX: 8 vWA: 15,17 |
| Tumor Formation: | Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells. |
| Products | epidermal keratins; 40 kD keratin |
| Biosafety | 1 |
| Addtional Info | SCC-4 forms colonies in semi-solid medium, and is not induced to differentiate by anchorage deprivation. |
| Culture Medium | 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium containing 1.2 g/L sodium bicarbonate, 2 mM L-glutamine, 400 ng/mL hydrocortisone and 10% of fetal bovine serum. |
| Subculturing | Note: Subculture before 100% confluent. Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Inoculate new flasks with 3 x 10e3 cells/cm2. Incubate cultures at 37°C. Population Doubling Time: 60-70 hours NOTE: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 12 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 6th edition, published by Alan R. Liss, N.Y., 2010. |
| Subculturing Medium Renewal | 1 to 2 times per week |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | Rheinwald JG, Beckett MA. Tumorigenic keratinocyte lines requiring anchorage and fibroblast support cultures from human squamous cell carcinomas. Cancer Res. 41: 1657-1663, 1981 |
| Depositors | ADELZON ASSIS DE PAULA; UFRJ |
| ATCC | CRL-1624 |
| Cellosaurus | CVCL_1684 |
