| BCRJ Code | 0344 |
| Cell Line | SJCRH30 [RC13, RMS 13, SJRH30] |
| Species | Homo sapiens |
| Vulgar Name | Human |
| Tissue | Muscle, Derived From Metastatic Site: Bone Marrow |
| Cell Type | Fibroblast |
| Morphology | Fibroblast |
| Disease | Rhabdomyosarcoma |
| Growth Properties | Adherent |
| Sex | Male |
| Age/Ethinicity | 17 Year / Caucasian |
| Derivation | The line was established from cells from the bone marrow of a child with rhabdomyosarcoma. Derived from metastatic site, bone marrow |
| Products | Genes Expressed: gli + (amplified 30 fold), N-myc + (not amplified) |
| Biosafety | 1 |
| Addtional Info | The cells show ultrastructural elements of primitive skeletal muscle differentiation. |
| Culture Medium | RPMI-1640 medium modified to contain 2 mM L-glutamine, 4500 mg/L glucose and 10% of fetal bovine serum. |
| Subculturing | Remove medium, and rinse with PBS without calcium and magnesium . Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. NOTE: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 12 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 6th edition, published by Alan R. Liss, N.Y., 2010. |
| Subculturing Medium Renewal | Twice per week |
| Subculturing Subcultivation Ratio | 1:5 to 1:10 is recommended |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Cryopreservation | 95% FBS + 5% DMSO (Dimethyl sulfoxide) |
| Thawing Frozen Cells | SAFETY PRECAUTION:
It is strongly recommended to always wear protective gloves, clothing, and a full-face mask when handling frozen vials. Some vials may leak when submerged in liquid nitrogen, allowing nitrogen to slowly enter the vial. Upon thawing, the conversion of liquid nitrogen back to its gas phase may cause the vial to explode or eject its cap with significant force, creating flying debris.
NOTE: It is important to avoid excessive alkalinity of the medium during cell recovery. To minimize this risk, it is recommended to place the culture vessel containing the growth medium in the incubator for at least 15 minutes before adding the vial contents. This allows the medium to stabilize at its normal pH (7.0 to 7.6). |
| References | Oliner JD, et al. Amplification of a gene encoding a p53-associated protein in human sarcomas. Nature 358: 80-83, 1992. PubMed: 1614537 Roberts WM, et al. Amplification of the gli gene in childhood sarcomas. Cancer Res. 49: 5407-5413, 1989. PubMed: 2766305 Douglass EC, et al. A specific chromosomal abnormality in rhabdomyosarcoma [published erratum appears in Cytogenet Cell Genet 1988;47(4):following 232]. Cytogenet. Cell Genet. 45: 148-155, 1987. PubMed: 3691179 |
| Depositors | Magnus Regios Dias da Silva - UNIFESP |
| Cellosaurus |
